Angelita Simonetti (IBMC Strasbourg), Patrick Bron (CBS Montpellier)
Keywords: macromolecular complexes ; single-particle analysis ; cryo-electron microscopy ; image processing ; 3D reconstruction
In recent years, cryo-electron microscopy (cryo-EM) has undergone a "resolution revolution" providing structures of a growing number of biological complexes at close to atomic resolution. Thirty years of evolution and progress, which have conferred to cryo-EM a predominant role in the structural investigation and have resulted in the attribution of the 2017 Nobel Prize in Chemistry to J. Dubochet, J. Franck and R. Henderson “for developing the cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution". Although cryo-EM has become a major tool in structural biology, the technique has not yet reached its full potential since some resolution limitations are still present (i.e. sample size and preparation, radiation damage, etc...).
This session aims to highlight recent developments in cryo-EM and its contribution to various biological issues.